Carcinogenic Potency Database Example of Published Plot Format

A sample of the plot of the CPDB and description for one experiment is given below.

Left side of the CPDB plot.

     Spe Strain  Site    Xpo+Xpt                                                                                   TD50     2Tailpvl
       Sex   Route   Hist      Notes                                                                                     DR     AuOp
 [1] [2][3][4][5] [6] [7] [8][9][10]                                      [11]                [12]                 [13] [14][15][16]

CHLOROFORM                        100ng..:..1ug....:..10.....:..100....:..1mg....:..10.....:..100....:..1g.....:..10
1401 R m osm gav kid MXA 18m26                                                               : +    :              119.mg / P<.0005c
 a   R m osm gav TBA MXB 18m26                                                                                     194.mg * P<.5
 b   R m osm gav liv MXB 18m26                                                                                     455.mg * P<.08

Right side of the CPDB plot.

     RefNum  LoConf UpConf   Cntrl  1Dose    1Inc  2Dose    2Inc                                Citation or Pathology
                                                                                                                          Brkly Code
 [17][18][19] [20]   [21]    [22]   [23]    [24]   [25]     [26]                                                  [27]

CHLOROFORM      67-66-3
1401   TR-A  65.5mg 334.mg   0/20   45.2mg   4/50  90.3mg  12/50                                                      kid:tla,uac.
 a     TR-A  43.5mg n.s.s.   9/20   45.2mg  24/50  90.3mg  20/50
 b     TR-A  157.mg n.s.s.   0/20   45.2mg   1/50  90.3mg   3/50                                                  liv:hpa,hpc,nnd.

A unique number is assigned to each experiment in the CPDB plot, and lowercase letters for subsequent lines identify each TD₅₀ calculated for that experiment. The number inserted above each field corresponds to the description below.

[1]

Chloroform is the chemical. The three asterisks indicate that chloroform results were reported in more than one published plot of the CPDB.

[2]

R: species is rat.

[3]

m: sex is male.

[4]

osm: strain is Osborne-Mendel.

[5]

gav: route of administration is gavage.

[6]

kid: site is kidney.

[7]

MXA: histopathology is a mix of tumor types combined by NCI. The pathology is indicated on the right side of the plot under [27] where the codes are “kid:tla,uac” indicating a mix of tubular-cell adenoma and tubular-cell adenocarcinoma. The site and histopathology is reported in the nomenclature used in the original published paper or technical report.

[8]

18m: length of exposure is 18 months.

[9]

26m: length of experiment is 26 months.

[10]

“Notes” is blank here but is used to describe particulars such as survival problems or variable dosing schedules.

[11]

Logarithmic scale for TD₅₀ and confidence limits.

[12]

The plotted TD₅₀ value with the symbol “+” indicating that the statistical significance of the TD₅₀ is p<0.01. The symbol colon indicates the 99% confidence limits for TD₅₀ and shows that TD₅₀ was calculated with lifetable data.

[13]

119.mg: value of TD₅₀ in milligrams/kilogram/day.

[14]

Shape of the dose-response curve, determined by the goodness-of-fit test for departure from linearity. The symbol “/” indicates significant departure from linearity with upward curvature.

[15]

p<.0005: two-tailed p-value associated with testing whether the slope of the dose-response curve is different from zero.

[16]

c: NCI opinion that chloroform was carcinogenic, inducing the tumors indicated under [6] and [7].

[17]

The right side of the plot lists the experiment number.

[18]

67-66-3: CAS registry number for chloroform.

[19]

TR-A: unique reference number for a paper or an NCI/NTP identification number.

[20-21]

65.5mg and 334.mg: lower and upper confidence limits for the TD₅₀ in milligrams/kilograms/day.

[22-26]

Proportion of animals with tumors in [6-7] and average daily dose-rate in milligrams/kilograms/day for the control group and each dose group. For NCI/NTP the denominator indicates the starting number of animals, and the numerator indicates the number of animals with tumors by the end of the experiment.

[27]

Codes for histopathology for NCI/NTP and literature citation for papers published in the general literature. Lines a and b under this experiment give results for mandatory sites that are always included in the database whenever data are available. TBA: all tumor-bearing animals; and liv: liver.

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Last updated: August 6, 2007